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產(chǎn)品概述
| 名稱(chēng) | HTR-8-Svneo (人絨毛膜滋養(yǎng)層細(xì)胞) (STR鑒定正確) |
| 別稱(chēng) | HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn |
| 種屬 | 人 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:3 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | The HTR-8-Svneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen. |
| 年齡（性別） | 女性；6-12周齡胚胎 |
| 組織來(lái)源 | 胎盤(pán) |
| 細(xì)胞類(lèi)型 | 轉(zhuǎn)化細(xì)胞系 |
| 生物安全等級(jí) | BSL-1 |
| 致瘤性 | No (tested in soft agar and nude mice). |
| 抗原表達(dá)情況 | cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha |
| 保藏機(jī)構(gòu) | ATCC; CRL-3271 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 12 D2S1338 23,24 D3S1358 16 D5S818 12 D7S820 12 D8S1179 12,15 D13S317 9,12 D16S539 11,13 D18S51 13 D19S433 14,15 D21S11 29,30 FGA 21,23 PentaD 9,12 PentaE 7,17 TH01 6,9.3 TPOX 8 vWA 13,18 D6S1043 14,19 D12S391 19 D2S441 11 -
STR鑒定圖
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參考文獻(xiàn)
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Trophoblast-derived miR-410-5p induces M2 macrophage polarization and mediates immunotolerance at the fetal-maternal interface by targeting the STAT1 signaling pathway(2024/01/04)
作者：Jing Yang, Longfei Li, Linlin Wang
期刊：Journal of Translational Medicine
DOI：10.1186/s12967-023-04831-y
影響因子 ：7.400
引用產(chǎn)品：CL-0765
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Calcium/calmodulin dependent protein kinase IV in trophoblast cells under insulin resistance: functional and metabolomic analyses(2023/06/29)
作者：Ling Li, Li Li, Ying Shao
期刊：MOLECULAR MEDICINE
DOI：10.1186/s10020-023-00669-8
影響因子 ：5.700
引用產(chǎn)品：CL-0765
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NANOG regulate the JAK/STAT3 pathway to promote trophoblast cell migration and epithelial-mesenchymal transition (EMT) in hypertensive disorders of pregnancy (HDP) through protein interaction with CDK1(2024/05/26)
作者：Jing Ma, Mingchang Liu, Zhuo Chen
期刊：AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
影響因子 ：3.600
引用產(chǎn)品：CL-0765
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AQP9 causes recurrent spontaneous abortion by inhibiting trophoblast cell EMT and invasion through the PI3K/AKT pathway(2023/08/21)
作者：Nana Ma, Bo Liu, Yufu Jin
期刊：BIOLOGY OF REPRODUCTION
影響因子 ：3.600
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Inhibition of the endoplasmic reticulum stress-associated IRE-1 pathway alleviates preterm birth(2024/03/26)
作者：Liyin Qiu, Hui Liu, Shali Chen
期刊：AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
影響因子 ：3.600
引用產(chǎn)品：CL-0765
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HNRNPC mediated m6A methylation of 5-Methyltetrahydrofolate-homocysteine methyltransferase and involved in the occurrence of recurrent spontaneous abortion(2023/10/04)
作者：Mengting Zhang, Nana Huang, Yue Gao
期刊：JOURNAL OF REPRODUCTIVE IMMUNOLOGY
影響因子 ：3.400
引用產(chǎn)品：CL-0765
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Establishment of a placental lncRNA-mRNA expression network for early-onset preeclampsia(2024/04/27)
作者：Ya Chen, Ying Zhang, Siyu Xie
期刊：BMC Pregnancy and Childbirth
DOI：10.1186/s12884-024-06481-4
影響因子 ：3.100
引用產(chǎn)品：CL-0765
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Mechanisms of YAP1-mediated trophoblast ferroptosis in recurrent pregnancy loss(2024/03/25)
作者：Liping Chen, Fangfang Dai, Yanjie Huang
期刊：JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
DOI：10.1007/s10815-024-03096-8
影響因子 ：3.100
引用產(chǎn)品：CL-0765
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MiR-135a-5p regulates window of implantation by suppressing pinopodes development and decidualization of endometrial stromal cells(2024/03/21)
作者：Yunan He, Ying Ju, Hui Lei
期刊：JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
DOI：10.1007/s10815-024-03088-8
影響因子 ：3.100
引用產(chǎn)品：CL-0765
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Circ_FOXP1 promotes the growth and survival of high glucose-treated human trophoblast cells through the regulation of miR-508-3p/SMAD family member 2 pathway(2022/09/28)
作者：Mingqun Li, Yuqin Huang, Hongli Xi
期刊：ENDOCRINE JOURNAL
影響因子 ：2.860
引用產(chǎn)品：CL-0765
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MicroRNA-206 Contributes to the Progression of Preeclampsia by Suppressing the Viability and Mobility of Trophocytes via the Inhibition of AGTR1(2023/10/31)
作者：WeiWei Mo, Jiaxi Jin, Xuexin Wang
期刊：PHYSIOLOGICAL RESEARCH
DOI：10.33549/physiolres.935131
影響因子 ：2.100
引用產(chǎn)品：CL-0765
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Knockdown of TXNIP alleviates gestational diabetes mellitus by activating autophagy to regulate cell proliferation and apoptosis in high glucose-treated trophoblasts(2023/12/19)
作者：Ying Gu, Minkai Cao, Yu Chen
期刊：Reproductive Biology
DOI：10.1016/j.repbio.2023.100841
影響因子 ：2.100
引用產(chǎn)品：CL-0765
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Protective Effects of Paeoniflorin Against Inflammation and Oxidative Stress in Rats with Gestational Diabetes Mellitus via Inhibiting the RhoA/ROCK Signaling Pathway(2024/01/02)
作者：Chengkun Yuan, Yan Gao, Jinglu Yu
期刊：Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy
DOI：10.1007/s43450-023-00505-4
影響因子 ：1.600
引用產(chǎn)品：CL-0765
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COLEC12 expression participates in trophoblast insulin resistance, and reverses celecoxib-mediated inhibition of COX2-PGE2 axis in gestational diabetes(2023/06/05)
作者：Beibei Jin, Mi Hu, Jie Chen
期刊：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
影響因子 ：0.600
引用產(chǎn)品：CL-0765
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