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DMS 114 (人肺癌細胞) (STR鑒定正確)
貨號：CL-0752
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價格： ￥1500
生長培養(yǎng)基：Waymouth's MB 752/1+10%FBS＋1% P/S
產(chǎn)品概述
產(chǎn)品信息
| 名稱 | DMS 114 (人肺癌細胞) (STR鑒定正確) |
| 別稱 | DMS-114; DMS114; Darmouth Medical School 114 |
| 種屬 | 人 |
| 生長特性 | 貼壁細胞 |
| 細胞形態(tài) | 上皮細胞樣 |
| 凍存條件 | 凍存液：55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認） |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:4 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來源文獻)
| 背景描述 | The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin. |
| 年齡（性別） | 男性；68歲 |
| 組織來源 | 肺 |
| 細胞類型 | 腫瘤細胞 |
| 腫瘤類型 | 肺癌細胞 |
| 生物安全等級 | BSL-1 |
| 倍增時間 | 3.8 days (PubMed=6266631) |
| 致瘤性 | Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| 抗原表達情況 | Leu 7 +; My23 +; CD11b + |
| 基因表達情況 | adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP) |
| 保藏機構 | ATCC; CRL-2066 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 10,11 D2S1338 17 D3S1358 14,17 D5S818 12 D7S820 10,11 D8S1179 12,13 D13S317 13 D16S539 12 D18S51 18 D19S433 13 D21S11 29 FGA 21 PentaD 11,13 PentaE 7,13 TH01 8,9.3 TPOX 8 vWA 16,17 D6S1043 12,17 D12S391 18 D2S441 10 -
STR鑒定圖
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參考文獻
文獻列表
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NDRG2 acts as a negative regulator of the progression of small-cell lung cancer through the modulation of the PTEN-AKT-mTOR signalling cascade(2024/03/25)
作者：Zhenchuan Ma, Yuefeng Ma, Jie Feng
期刊：TOXICOLOGY AND APPLIED PHARMACOLOGY
DOI：10.1016/j.taap.2024.116915
影響因子 ：3.800
引用產(chǎn)品：CL-0752
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