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產(chǎn)品概述
產(chǎn)品信息
| 別名 | Catabolin, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endoge_x0002_nous Mediator (LEM), Mononuclear Cell Factor (MCF) |
| 物種 | Mouse |
| 表達(dá)宿主 | E.coli |
| 序列信息 | MVPIRQLHYRLRDEQQKSLVLSDPYELKALHLNGQNINQQVIFSMSFVQGEPSNDKIPVALGLKGKNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKSKVEFESAEFPNWYISTSQAEHKPVFLGNNSGQDIIDFTMESVSS with polyhistidine tag at the C-terminus. |
| 檢索號 | P10749.1 |
| 分子量 | 18.3 kDa |
| 表觀分子量 | 17 kDa |
| 標(biāo)簽 | His-tag?at?the?C-terminus |
| 生物活性 | Measure by its ability to induce D10.G4.1 cells proliferation.The ED50 for this effect is <8 pg/mL.The specific activity of recombinant mouse IL-1 beta is approximately >1.2x 10^8 IU/mg. |
產(chǎn)品特性
| 純度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 內(nèi)毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 運(yùn)輸 | Ambient temperature or ice pack. |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹
Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β.
參考文獻(xiàn)
文獻(xiàn)列表
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Formononetin Inhibits Microglial Inflammatory Response and Contributes to Spinal Cord Injury Repair by Targeting the EGFR/MAPK Pathway(2023/03/28)
作者：Haiping Fu, Mingdong Li, Yanqiang Huan
期刊：IMMUNOLOGICAL INVESTIGATIONS
DOI：10.1080/08820139.2023.2183135
影響因子 ：2.800
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