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產(chǎn)品概述
產(chǎn)品信息
| 名稱 | GP2d (人結(jié)腸癌細(xì)胞) (STR鑒定正確) |
| 別稱 | Gp2d; Gp2D; GP2D |
| 種屬 | 人 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:4 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來源文獻(xiàn))
| 背景描述 | GP2d has been established from the same adenocarcinoma as GP5d (ECACC catalogue no. 95090715). This has been confirmed by STR profiling at ECACC. The cells were derived from a local recurrence of Duke's grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection. The patient received no preoperative chemo- or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years. Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF alpha or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d. |
| 年齡（性別） | 女性；71歲 |
| 組織來源 | 結(jié)腸 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 結(jié)腸癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | 48-60hours |
| 保藏機(jī)構(gòu) | ECACC; 95090714 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 9,12 D2S1338 18 D3S1358 16,18 D5S818 11,12 D7S820 9,11 D8S1179 10,12 D13S317 8,12 D16S539 11,13 D18S51 12,13 D19S433 12,13 D21S11 29,30 FGA 23 PentaD 12,13 PentaE 7,10 TH01 7,9.3 TPOX 8,11 vWA 17,18,19 D6S1043 10,17 D12S391 19,22,23 D2S441 10,11 -
STR鑒定圖
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