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產(chǎn)品概述
產(chǎn)品信息
| 別名 | T-cell Growth Factor (TCGF), Aldesleukin |
| 物種 | Mouse |
| 表達宿主 | E.coli |
| 序列信息 | MAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ with polyhistidine tag at the C-terminus. |
| 檢索號 | P04351.1 |
| 分子量 | 18.2 kDa |
| 表觀分子量 | 18 kDa |
| 標(biāo)簽 | His-tag?at?the?C-terminus |
| 生物活性 | Measure by its ability to induce CTLL-2 cells proliferation.The ED50 for this effect is <0.3 ng/mL.The specific activity of recombinant mouse IL-2 is approximately >3x10^6 IU/mg. |
產(chǎn)品特性
| 純度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 內(nèi)毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 運輸 | Ambient temperature or ice pack. |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
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