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產品概述
產品信息
別名 | AI118077, D3Ertd299, D3Ertd299e |
物種 | Mouse |
表達宿主 | E.coli |
序列信息 | MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL with polyhis |
檢索號 | P51859.2 |
分子量 | 27.08 kDa |
表觀分子量 | 35-48 kDa |
標簽 | His-tag?at?the?C-terminus |
產品特性
純度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
內毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
運輸 | Ambient temperature or ice pack. |
制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4. |
復融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹
HDGF is a member of the hepatoma-derived growth factor family. HDGF plays a role as a secreted mitogen from the human hepatoma cell line Huh-7. HDGF is a nuclear targeted vascular smooth muscle cell mitogen as well as a heparin-binding protein that is greatly expressed in tumor cells where it stimulates proliferation. HDGF has several biochemical functions, for example, DNA binding, RNA polymerase II transcription corepressor activity, growth factor activity. Some of the functions are cooperated with other proteins, some of the functions could acted by HDGF itself.
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